GENETIC POLYMORPHISM ANALYSIS OF AHR2 GENE IN CYPRINUS CARPIO AFTER EXPOSURE TO BENZO (GHI) PERYLENE AND BENZO(A)PYRENE

Abstract

There is growing concern about the presence of mutagenic and carcinogenic pollutants in the aquatic environment as they pose a great risk to the health of aquatic organisms, and pollution of aquatic ecosystems is one of the main risk factors for human health. Polycyclic aromatic hydrocarbons (PAHs) are one of the most influential and prevalent organic pollutants. One of the main receptors that represents the main pathway to stimulating the metabolism of these pollutants is the aryl hydrocarbon receptor (AhR2). This study is the first to detect the mutational ability of benzo(a)pyrene B(a)P and benzo(ghi)pyrene B(ghi)P compounds in the AhR2 gene in common carp fish. 30 common carp weighing an average of 35 g were divided into three groups: The first group, the control, was not given any of the test solutions, while the second group was given three doses of B(a)P with a concentration of 10μg/kg, and the third group was given three doses of B(ghi)P with a concentration of 10μg/kg. The results revealed B(a)P's high ability to mutate, causing 13 mutations in the amplified part of the AhR2 gene and 12 changes in amino acids, leading to the emergence of four new haplotypes. B(ghi)P's mutational ability was also detected, but it was less than that of B(a)P as it caused only four mutations and three changes in amino acids and the emergence of four new haplotypes. The installation of three-dimensional proteins has also changed in both groups.